A modified staining technique for arbuscular mycorrhiza compatible with molecular probes.
Identifieur interne : 002B30 ( Main/Exploration ); précédent : 002B29; suivant : 002B31A modified staining technique for arbuscular mycorrhiza compatible with molecular probes.
Auteurs : M. Pitet [Espagne] ; A. Camprubí [Espagne] ; C. Calvet [Espagne] ; V. Estaún [Espagne]Source :
- Mycorrhiza [ 0940-6360 ] ; 2009.
Descripteurs français
- KwdFr :
- ADN fongique (génétique), ADN fongique (isolement et purification), ADN ribosomique (génétique), ADN ribosomique (isolement et purification), Champignons (génétique), Coloration et marquage (méthodes), Mycorhizes (génétique), Racines de plante (microbiologie), Sensibilité et spécificité (MeSH), Sondes moléculaires (génétique).
- MESH :
- génétique : ADN fongique, ADN ribosomique, Champignons, Mycorhizes, Sondes moléculaires.
- isolement et purification : ADN fongique, ADN ribosomique.
- microbiologie : Racines de plante.
- méthodes : Coloration et marquage.
- Sensibilité et spécificité.
English descriptors
- KwdEn :
- DNA, Fungal (genetics), DNA, Fungal (isolation & purification), DNA, Ribosomal (genetics), DNA, Ribosomal (isolation & purification), Fungi (genetics), Molecular Probes (genetics), Mycorrhizae (genetics), Plant Roots (microbiology), Sensitivity and Specificity (MeSH), Staining and Labeling (methods).
- MESH :
- chemical , genetics : DNA, Fungal, DNA, Ribosomal, Molecular Probes.
- chemical , isolation & purification : DNA, Fungal, DNA, Ribosomal.
- genetics : Fungi, Mycorrhizae.
- methods : Staining and Labeling.
- microbiology : Plant Roots.
- Sensitivity and Specificity.
Abstract
The effects of the different steps of the root staining on the arbuscular mycorrhizal (AM) fungal rDNA extraction and amplification have been assessed. The results obtained using molecular techniques are compared with those obtained from fresh, non-stained leek roots. A modified staining procedure that eliminates heating, the use of hydrochloric acid and trypan blue, has been proved to be the most adequate to observe the AM colonisation in different plant species with/without lignified roots allowing at the same time the subsequent rDNA extraction and amplification from the stained roots. The staining technique decreased the sensitivity of the process and a higher number of roots had to be used to obtain enough material for a positive amplification. The extraction and amplification process was reliable up to 3 days after staining. A week after staining, the amplification was not dependable and after 2 weeks there was no amplification from stained material.
DOI: 10.1007/s00572-008-0206-1
PubMed: 18958505
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Calvet</name><affiliation wicri:level="3"><nlm:affiliation>Plant Protection IRTA, Ctra. de Cabrils Km 2, E-08348 Cabrils, Barcelona, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Plant Protection IRTA, Ctra. de Cabrils Km 2, E-08348 Cabrils, Barcelona</wicri:regionArea><placeName><settlement type="city">Barcelone</settlement><region nuts="2" type="region">Catalogne</region></placeName></affiliation></author><author><name sortKey="Estaun, V" sort="Estaun, V" uniqKey="Estaun V" first="V" last="Estaún">V. Estaún</name><affiliation wicri:level="3"><nlm:affiliation>Plant Protection IRTA, Ctra. de Cabrils Km 2, E-08348 Cabrils, Barcelona, Spain. victoria.estaun@irta.cat.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Plant Protection IRTA, Ctra. de Cabrils Km 2, E-08348 Cabrils, Barcelona</wicri:regionArea><placeName><settlement type="city">Barcelone</settlement><region nuts="2" type="region">Catalogne</region></placeName></affiliation></author></analytic><series><title level="j">Mycorrhiza</title><idno type="ISSN">0940-6360</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>DNA, Fungal (genetics)</term><term>DNA, Fungal (isolation & purification)</term><term>DNA, Ribosomal (genetics)</term><term>DNA, Ribosomal (isolation & purification)</term><term>Fungi (genetics)</term><term>Molecular Probes (genetics)</term><term>Mycorrhizae (genetics)</term><term>Plant Roots (microbiology)</term><term>Sensitivity and Specificity (MeSH)</term><term>Staining and Labeling (methods)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN fongique (génétique)</term><term>ADN fongique (isolement et purification)</term><term>ADN ribosomique (génétique)</term><term>ADN ribosomique (isolement et purification)</term><term>Champignons (génétique)</term><term>Coloration et marquage (méthodes)</term><term>Mycorhizes (génétique)</term><term>Racines de plante (microbiologie)</term><term>Sensibilité et spécificité (MeSH)</term><term>Sondes moléculaires (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Fungal</term><term>DNA, Ribosomal</term><term>Molecular Probes</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>DNA, Fungal</term><term>DNA, Ribosomal</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Fungi</term><term>Mycorrhizae</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN fongique</term><term>ADN ribosomique</term><term>Champignons</term><term>Mycorhizes</term><term>Sondes moléculaires</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>ADN fongique</term><term>ADN ribosomique</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Staining and Labeling</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Racines de plante</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Plant Roots</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Coloration et marquage</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Sensibilité et spécificité</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The effects of the different steps of the root staining on the arbuscular mycorrhizal (AM) fungal rDNA extraction and amplification have been assessed. The results obtained using molecular techniques are compared with those obtained from fresh, non-stained leek roots. A modified staining procedure that eliminates heating, the use of hydrochloric acid and trypan blue, has been proved to be the most adequate to observe the AM colonisation in different plant species with/without lignified roots allowing at the same time the subsequent rDNA extraction and amplification from the stained roots. The staining technique decreased the sensitivity of the process and a higher number of roots had to be used to obtain enough material for a positive amplification. The extraction and amplification process was reliable up to 3 days after staining. A week after staining, the amplification was not dependable and after 2 weeks there was no amplification from stained material.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">18958505</PMID><DateCompleted><Year>2009</Year><Month>03</Month><Day>24</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0940-6360</ISSN><JournalIssue CitedMedium="Print"><Volume>19</Volume><Issue>2</Issue><PubDate><Year>2009</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Mycorrhiza</Title><ISOAbbreviation>Mycorrhiza</ISOAbbreviation></Journal><ArticleTitle>A modified staining technique for arbuscular mycorrhiza compatible with molecular probes.</ArticleTitle><Pagination><MedlinePgn>125-131</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s00572-008-0206-1</ELocationID><Abstract><AbstractText>The effects of the different steps of the root staining on the arbuscular mycorrhizal (AM) fungal rDNA extraction and amplification have been assessed. The results obtained using molecular techniques are compared with those obtained from fresh, non-stained leek roots. A modified staining procedure that eliminates heating, the use of hydrochloric acid and trypan blue, has been proved to be the most adequate to observe the AM colonisation in different plant species with/without lignified roots allowing at the same time the subsequent rDNA extraction and amplification from the stained roots. The staining technique decreased the sensitivity of the process and a higher number of roots had to be used to obtain enough material for a positive amplification. The extraction and amplification process was reliable up to 3 days after staining. A week after staining, the amplification was not dependable and after 2 weeks there was no amplification from stained material.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Pitet</LastName><ForeName>M</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Plant Protection IRTA, Ctra. de Cabrils Km 2, E-08348 Cabrils, Barcelona, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Camprubí</LastName><ForeName>A</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Plant Protection IRTA, Ctra. de Cabrils Km 2, E-08348 Cabrils, Barcelona, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Calvet</LastName><ForeName>C</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Plant Protection IRTA, Ctra. de Cabrils Km 2, E-08348 Cabrils, Barcelona, Spain.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Estaún</LastName><ForeName>V</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>Plant Protection IRTA, Ctra. de Cabrils Km 2, E-08348 Cabrils, Barcelona, Spain. victoria.estaun@irta.cat.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D023362">Evaluation Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2008</Year><Month>10</Month><Day>29</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Mycorrhiza</MedlineTA><NlmUniqueID>100955036</NlmUniqueID><ISSNLinking>0940-6360</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004271">DNA, Fungal</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D004275">DNA, Ribosomal</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D015335">Molecular Probes</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D004271" MajorTopicYN="N">DNA, Fungal</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004275" MajorTopicYN="N">DNA, Ribosomal</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005658" MajorTopicYN="N">Fungi</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015335" MajorTopicYN="N">Molecular Probes</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D038821" MajorTopicYN="N">Mycorrhizae</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018517" MajorTopicYN="N">Plant Roots</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="N">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013194" MajorTopicYN="N">Staining and Labeling</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2008</Year><Month>05</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2008</Year><Month>10</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2008</Year><Month>10</Month><Day>30</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>3</Month><Day>25</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2008</Year><Month>10</Month><Day>30</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">18958505</ArticleId><ArticleId IdType="doi">10.1007/s00572-008-0206-1</ArticleId><ArticleId IdType="pii">10.1007/s00572-008-0206-1</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Mycorrhiza. 2004 Apr;14(2):111-7</Citation><ArticleIdList><ArticleId 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IdType="pubmed">15548818</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Espagne</li></country><region><li>Catalogne</li></region><settlement><li>Barcelone</li></settlement></list><tree><country name="Espagne"><region name="Catalogne"><name sortKey="Pitet, M" sort="Pitet, M" uniqKey="Pitet M" first="M" last="Pitet">M. Pitet</name></region><name sortKey="Calvet, C" sort="Calvet, C" uniqKey="Calvet C" first="C" last="Calvet">C. Calvet</name><name sortKey="Camprubi, A" sort="Camprubi, A" uniqKey="Camprubi A" first="A" last="Camprubí">A. Camprubí</name><name sortKey="Estaun, V" sort="Estaun, V" uniqKey="Estaun V" first="V" last="Estaún">V. Estaún</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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